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KMID : 1149220130210020063
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Korean Journal of Oriental Medical Prescription
2013 Volume.21 No. 2 p.63 ~ p.71
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Cytoprotective Effects of Artemisia princeps Extract through Inhibition of Mitochondrial Dysfunction
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Choi Hee-Yoon
Jeggal Kyung-Hwan
Kim Young-Woo
Lee Jung-Woo
Jo Soo-A
Cho Il-Je
Kim Sang-Chan
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Abstract
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Objectives : Artemisia princeps is used as moxa in moxibustion and traditional herbal medicine. And its extracts or compounds is known to have an efficacy of antioxidant, anti-diabete, anti-cancer, anti-inflammation and neuroprotection. This study was performed to investigate the cytoprotective effect of Artemisia princeps extract (APE) against arachidonic acid (AA)+iron-induced oxidative stress on HepG2 cell.
Methods : The effects of APE on cell viability has been assessed using MTT assay. And flow cytometric analysis was performed to estimate APE's effects on mitochondrial function. To investigate its underlying mechanism, related protein was analysed by using immunoblot analysis.
Results : Treatment of APE increased relative cell viability, prevented a decline of B-cell lymphoma-extra large (Bcl-xL) and cleavage of poly(ADP-ribose) polymerase (PARP) and procaspase-3, and also protected mitochondrial membrane permeability (MMP) against oxidative stress induced by AA+iron. In addition, APE treatment increased phosphorylation of AMP-activated protein kinase (AMPK) exerts a cytoprotective effect.
Conclusions : This results demonstrate that APE has an ability to activation of AMPK which protects cells from AA+iron-induced oxidative stress and restores MMP.
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KEYWORD
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Artemisia princeps, Iron, Arachinodic acid, AMPK, Oxidative stress
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